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You searched for: EV180076 (EV-TRACK ID)
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Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Isolation protocol | First author | Year | EV-METRIC |
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EV180076 | 1/2 | Mus musculus | Bone marrow supernatant | Commercial method | Singleton Q | 2019 | 25% | |
Study summaryFull title
All authors
Singleton Q, Vaibhav K, Braun M, Patel C, Khayrullin A, Mendhe B, Lee BR, Kolhe R, Kaiser H, Awad ME, Fariyike T, Elsayed R, Elsalanty M, Isales CM, Liu Y, Hamrick MW, Dhandapani KM, Fulzele S.
Journal
Cells
Abstract
Traumatic brain injury (TBI) is a major source of worldwide morbidity and mortality. Patients suffer (show more...)
EV-METRIC
25% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Isolation method: density gradient, at least as validation of results attributed to EVs
EV density
Isolation method: reporting of obtained EV density
ultracentrifugation specifics
Isolation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bone marrow supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Isolation protocol
Isolation protocol
Commercial method
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Bone marrow supernatant
Sample Condition
Control condition
Isolation Method
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD63/ TSG101
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
100
EV concentration
Yes
EM
EM-type
Transmission-EM/ Immuno-EM
Image type
Close-up, Wide-field
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EV180076 | 2/2 | Mus musculus | Bone marrow supernatant | Commercial method | Singleton Q | 2019 | 0% | |
Study summaryFull title
All authors
Singleton Q, Vaibhav K, Braun M, Patel C, Khayrullin A, Mendhe B, Lee BR, Kolhe R, Kaiser H, Awad ME, Fariyike T, Elsayed R, Elsalanty M, Isales CM, Liu Y, Hamrick MW, Dhandapani KM, Fulzele S.
Journal
Cells
Abstract
Traumatic brain injury (TBI) is a major source of worldwide morbidity and mortality. Patients suffer (show more...)
EV-METRIC
0% (median: 12% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Isolation method: density gradient, at least as validation of results attributed to EVs
EV density
Isolation method: reporting of obtained EV density
ultracentrifugation specifics
Isolation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bone marrow supernatant
Sample origin
Traumatic brain injury
Focus vesicles
extracellular vesicle
Isolation protocol
Isolation protocol
Commercial method
Protein markers
EV: TSG101/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Bone marrow supernatant
Sample Condition
Traumatic brain injury
Isolation Method
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD63/ TSG101
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
100
EV concentration
Yes
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