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You searched for: EV170000 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV170000 | 1/2 | Mus musculus | Serum |
(d)(U)C Filtration |
Thomou T | 2017 | 29% | |
Study summaryFull title
All authors
Thomou T, Mori MA, Dreyfuss JM, Konishi M, Sakaguchi M, Wolfrum C, Rao TN, Winnay JN, Garcia-Martin R, Grinspoon SK, Gorden P, Kahn
Journal
Nature
Abstract
Adipose tissue is a major site of energy storage and has a role in the regulation of metabolism thro (show more...)
EV-METRIC
29% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NA
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
EM
EM-type
Transmission-EM/ Immune-EM
EM protein
CD63;CD9
Image type
Close-up, Wide-field
Report size (nm)
80-200
Other particle analysis name(1)
EXOCET ELISA assay
EV-concentration
Yes
|
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EV170000 | 2/2 | Homo sapiens | Serum |
(d)(U)C Filtration |
Thomou T | 2017 | 0% | |
Study summaryFull title
All authors
Thomou T, Mori MA, Dreyfuss JM, Konishi M, Sakaguchi M, Wolfrum C, Rao TN, Winnay JN, Garcia-Martin R, Grinspoon SK, Gorden P, Kahn
Journal
Nature
Abstract
Adipose tissue is a major site of energy storage and has a role in the regulation of metabolism thro (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NA
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: speed (g)
100000
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
Other particle analysis name(1)
EXOCET ELISA assay
EV-concentration
Yes
|
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1 - 2 of 2 |
EV-TRACK ID | EV170000 | |
---|---|---|
species | Mus musculus | Homo sapiens |
sample type | Serum | Serum |
separation protocol | (d)(U)C Filtration | (d)(U)C Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 29 | 0 |