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You searched for: EV150090 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150090 | 1/3 | Homo sapiens | NAY | (d)(U)C | Shi R | 2015 | 0% | |
Study summaryFull title
All authors
Shi R, Wang PY, Li XY, Chen JX, Li Y, Zhang XZ, Zhang CG, Jiang T, Li WB, Ding W, Cheng SJ
Journal
Oncotarget
Abstract
Glioma is a most common type of primary brain tumors. Extracellular vesicles, in the form of exosome (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV150090 | 2/3 | Homo sapiens | Serum | (d)(U)C | Shi R | 2015 | 0% | |
Study summaryFull title
All authors
Shi R, Wang PY, Li XY, Chen JX, Li Y, Zhang XZ, Zhang CG, Jiang T, Li WB, Ding W, Cheng SJ
Journal
Oncotarget
Abstract
Glioma is a most common type of primary brain tumors. Extracellular vesicles, in the form of exosome (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD63
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV150090 | 3/3 | Homo sapiens | "Cerebrospinal fluid" | (d)(U)C | Shi R | 2015 | 0% | |
Study summaryFull title
All authors
Shi R, Wang PY, Li XY, Chen JX, Li Y, Zhang XZ, Zhang CG, Jiang T, Li WB, Ding W, Cheng SJ
Journal
Oncotarget
Abstract
Glioma is a most common type of primary brain tumors. Extracellular vesicles, in the form of exosome (show more...)
EV-METRIC
0% (median: 18% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
"Cerebrospinal fluid"
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD63
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
1 - 3 of 3 |
EV-TRACK ID | EV150090 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | Serum | Cerebrospinal fluid |
cell type | NAY | NA | NA |
medium | EV Depleted | ||
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C | (d)(U)C | (d)(U)C |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 0 | 0 | 0 |