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You searched for: EV150058 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150058 | 1/1 | Mus musculus | DNF | (d)(U)C | Zhang L | 2015 | 11% | |
Study summaryFull title
All authors
Zhang L, Zhang S, Yao J, Lowery FJ, Zhang Q, Huang WC, Li P, Li M, Wang X, Zhang C, Wang H, Ellis K, Cheerathodi M, McCarty JH, Palmieri D, Saunus J, Lakhani S, Huang S, Sahin AA, Aldape KD, Steeg PS, Yu D
Journal
Nature
Abstract
The development of life-threatening cancer metastases at distant organs requires disseminated tumour (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ TSG101/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
DNF
EV-harvesting Medium
EV Depleted
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Density gradient
Density medium
DNF
Pelleting: adjusted k-factor
DNF
Pelleting-wash: rotor type
DNF
Pelleting-wash: adjusted k-factor
DNF
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ TSG101
Proteomics database
DNF
Characterization: Lipid analysis
DNF
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
EV concentration
DNF
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EV-TRACK ID | EV150058 |
---|---|
species | Mus musculus |
sample type | Cell culture |
separation protocol | (d)(U)C |
Exp. nr. | 1 |
EV-METRIC % | 11 |