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You searched for: EV150014 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150014 | 1/2 | Homo sapiens | NAY |
(d)(U)C Filtration SEC UF |
Grasso L | 2015 | 33% | |
Study summaryFull title
All authors
Grasso L, Wyss R, Weidenauer L, Thampi A, Demurtas D, Prudent M, Lion N, Vogel H
Journal
Anal Bioanal Chem
Abstract
We report on a generic method to detect and identify the molecular profile of exosomes either derive (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration SEC UF Protein markers
EV: CD44/ EpCAM/ CD63/ CD9/ CD24
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD44/ CD24/ EpCAM
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD44/ CD24/ EpCAM
Characterization: Particle analysis
DLS
EM
EM-type
cryo EM
Image type
Close-up
|
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EV150014 | 2/2 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Grasso L | 2015 | 33% | |
Study summaryFull title
All authors
Grasso L, Wyss R, Weidenauer L, Thampi A, Demurtas D, Prudent M, Lion N, Vogel H
Journal
Anal Bioanal Chem
Abstract
We report on a generic method to detect and identify the molecular profile of exosomes either derive (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD44/ CD63/ CD9/ CD24
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD44/ CD24
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD44/ CD24
Characterization: Particle analysis
DLS
EM
EM-type
cryo EM
Image type
Close-up
|
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1 - 2 of 2 |
EV-TRACK ID | EV150014 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | Blood plasma |
cell type | NAY | NA |
medium | serum free | |
condition | NAY | NAY |
separation protocol | (d)(U)C Filtration SEC UF | (d)(U)C Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 33 | 33 |