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You searched for: EV150013 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150013 | 1/1 | Mus musculus | DNF |
(d)(U)C ExoQuick Filtration |
Zhu Y | 2015 | 33% | |
Study summaryFull title
All authors
Zhu Y, Chen X, Pan Q, Wang Y, Su S, Jiang C, Li Y, Xu N, Wu L, Lou X, Liu S
Journal
J Proteome Res
Abstract
Exosomes are 30-120 nm-sized membrane vesicles of endocytic origin that are released into the extrac (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration Protein markers
EV: Alix/ TSG101/ HSP90
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
DNF
EV-harvesting Medium
serum free
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Density medium
DNF
Pelleting: adjusted k-factor
DNF
Pelleting-wash: rotor type
DNF
Pelleting-wash: adjusted k-factor
DNF
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ HSP90/ TSG101
Detected contaminants
Cell organelle protein
Proteomics database
DNF
Characterization: Lipid analysis
DNF
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
EV concentration
DNF
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EV-TRACK ID | EV150013 |
---|---|
species | Mus musculus |
sample type | Cell culture |
separation protocol | (d)(U)C ExoQuick Filtration |
Exp. nr. | 1 |
EV-METRIC % | 33 |