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You searched for: EV140193 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140193 | 2/3 | Homo sapiens | Semen |
(d)(U)C ExoQuick IAF |
Madison MN | 2014 | 38% | |
Study summaryFull title
All authors
Madison MN, Roller RJ, Okeoma CM
Journal
Retrovirology
Abstract
BACKGROUND: Exosomes are membranous nanovesicles secreted into the extracellular milieu by diverse c (show more...)
EV-METRIC
38% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick IAF Protein markers
EV: CD81/ CD63/ GAPDH
non-EV: Cell organelle protein Proteomics
no
TEM measurements
50-100
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoQuick
Immunoaffinity capture
Selected surface protein(s)
MHC2
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ GAPDH
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
GAPDH
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up
Report size (nm)
50-100
|
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EV140193 | 3/3 | Homo sapiens | Semen |
(d)(U)C DG Filtration |
Madison MN | 2014 | 29% | |
Study summaryFull title
All authors
Madison MN, Roller RJ, Okeoma CM
Journal
Retrovirology
Abstract
BACKGROUND: Exosomes are membranous nanovesicles secreted into the extracellular milieu by diverse c (show more...)
EV-METRIC
29% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
255.8 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Density gradient
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Particle analysis
None
|
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EV140193 | 1/3 | Homo sapiens | Serum | ExoQuick | Madison MN | 2014 | 0% | |
Study summaryFull title
All authors
Madison MN, Roller RJ, Okeoma CM
Journal
Retrovirology
Abstract
BACKGROUND: Exosomes are membranous nanovesicles secreted into the extracellular milieu by diverse c (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Particle analysis
None
|
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1 - 3 of 3 |
EV-TRACK ID | EV140193 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Semen | Semen | Serum |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C ExoQuick IAF | (d)(U)C DG Filtration | ExoQuick |
Exp. nr. | 2 | 3 | 1 |
EV-METRIC % | 38 | 29 | 0 |