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You searched for: EV140045 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140045 | 2/2 | Homo sapiens | NAY |
(d)(U)C DG |
Webber J | 2014 | 38% | |
Study summaryFull title
All authors
Webber J, Stone TC, Katilius E, Smith BC, Gordon B, Mason MD, Tabi Z, Brewis IA, Clayton A
Journal
Mol Cell Proteomics
Abstract
We have used a novel affinity-based proteomics technology to examine the protein signature of small (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: CD81/ PSMA/ CD63/ CD9/ MHC1
non-EV: Proteomics
yes
EV density (g/ml)
1.12-1.17
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Lowest density fraction
0.2
Highest density fraction
2.5
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
PSMA/ MHC1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ CD9/ PSMA/ MHC1
Characterization: Particle analysis
NTA
|
||||||||
EV140045 | 1/2 | Homo sapiens | NAY |
(d)(U)C DC |
Webber J | 2014 | 33% | |
Study summaryFull title
All authors
Webber J, Stone TC, Katilius E, Smith BC, Gordon B, Mason MD, Tabi Z, Brewis IA, Clayton A
Journal
Mol Cell Proteomics
Abstract
We have used a novel affinity-based proteomics technology to examine the protein signature of small (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: CD81/ TSG101/ CD63/ CD9/ L1CAM
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ CD9/ TSG101/ L1CAM
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ CD9/ TSG101/ L1CAM
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
None
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1 - 2 of 2 |
EV-TRACK ID | EV140045 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | NAY | |
condition | NAY | |
separation protocol | (d)(U)C DG | (d)(U)C DC |
Exp. nr. | 2 | 1 |
EV-METRIC % | 38 | 33 |