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You searched for: EV130262 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130262 | 3/3 | Homo sapiens | Blood plasma |
DC (d)(U)C |
Haderk F | 2013 | 29% | |
Study summaryFull title
All authors
Haderk F, Hanna B, Richter K, Schnölzer M, Zenz T, Stilgenbauer S, Lichter P, Seiffert M.
Journal
Leuk Lymphoma
Abstract
Extracellular vesicles (EVs) are membrane-enclosed nanoparticles 30 to 1000 nm in size and represent (show more...)
EV-METRIC
29% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
chronic lymphocytic leukemia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DC
(d)(U)C Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: time (min)
120
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
|
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EV130262 | 1/3 | Homo sapiens | primary PBMCs (chronic lymphocytic leukemia) and irradiated BHK-huCD40L coculture |
DC (d)(U)C |
Haderk F | 2013 | 14% | |
Study summaryFull title
All authors
Haderk F, Hanna B, Richter K, Schnölzer M, Zenz T, Stilgenbauer S, Lichter P, Seiffert M.
Journal
Leuk Lymphoma
Abstract
Extracellular vesicles (EVs) are membrane-enclosed nanoparticles 30 to 1000 nm in size and represent (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DC
(d)(U)C Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary PBMCs (chronic lymphocytic leukemia) and irradiated BHK-huCD40L coculture
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Cell viability (%)
90
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: time (min)
120
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
|
||||||||
EV130262 | 2/3 | Homo sapiens | BHK-CD40L |
DC (d)(U)C |
Haderk F | 2013 | 0% | |
Study summaryFull title
All authors
Haderk F, Hanna B, Richter K, Schnölzer M, Zenz T, Stilgenbauer S, Lichter P, Seiffert M.
Journal
Leuk Lymphoma
Abstract
Extracellular vesicles (EVs) are membrane-enclosed nanoparticles 30 to 1000 nm in size and represent (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Irradiated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DC
(d)(U)C Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
BHK-CD40L
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Cell viability (%)
90
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: time (min)
120
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
None
Protein Concentration Method
microBCA
Characterization: Lipid analysis
No
|
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1 - 3 of 3 |
EV-TRACK ID | EV130262 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Blood plasma | Cell culture | Cell culture |
cell type | NA | primary PBMCs (chronic lymphocytic leukemia) and irradiated BHK-huCD40L coculture | BHK-CD40L |
medium | NA | EV-depleted medium | EV-depleted medium |
condition | chronic lymphocytic leukemia | Control condition | Irradiated |
separation protocol | DC (d)(U)C | DC (d)(U)C | DC (d)(U)C |
Exp. nr. | 3 | 1 | 2 |
EV-METRIC % | 29 | 14 | 0 |