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You searched for: EV130226 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130226 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration UF |
Quintero IB | 2013 | 22% | |
Study summaryFull title
All authors
Quintero IB, Herrala AM, Araujo CL, Pulkka AE, Hautaniemi S, Ovaska K, Pryazhnikov E, Kulesskiy E, Ruuth MK, Soini Y, Sormunen RT, Khirug L, Vihko PT
Journal
PLoS One
Abstract
The molecular mechanisms underlying prostate carcinogenesis are poorly understood. Prostatic acid ph (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
128.7 (pelleting) / 128.7 (washing)
Protein markers
EV: Flotillin/ CD13/ Snapin
non-EV: Proteomics
no
Show all info
Study aim
Other/function of a protein (TMPAP) in prostate cancer development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
50Ti
Pelleting: adjusted k-factor
128.7
Wash: volume per pellet (ml)
1
Wash: Rotor Type
50Ti
Wash: adjusted k-factor
128.7
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD13/ Flotillin/ Snapin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD13/ Flotillin/ Snapin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV130226 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 22 |