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You searched for: EV130139 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130139 | 1/3 | Homo sapiens | "Cerebrospinal fluid" | (d)(U)C | Carone C | 2013 | 29% | |
Study summaryFull title
All authors
Carone C, Genedani S, Leo G, Filaferro M, Fuxe K, Agnati LF
Journal
Mol Ther Nucleic Acids
Abstract
Development of biofluid-based molecular diagnostic tests for cancer is an important step towards tum (show more...)
EV-METRIC
29% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
"Cerebrospinal fluid"
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
80
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ cryo EM
Image type
Close-up, Wide-field
|
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EV130139 | 2/3 | Homo sapiens | Serum |
(d)(U)C Filtration |
Carone C | 2013 | 0% | |
Study summaryFull title
All authors
Carone C, Genedani S, Leo G, Filaferro M, Fuxe K, Agnati LF
Journal
Mol Ther Nucleic Acids
Abstract
Development of biofluid-based molecular diagnostic tests for cancer is an important step towards tum (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
80
Filtration steps
> 0.45 µm,
Characterization: Particle analysis
NTA
|
||||||||
EV130139 | 3/3 | Homo sapiens | Blood plasma | Carone C | 2013 | 0% | ||
Study summaryFull title
All authors
Carone C, Genedani S, Leo G, Filaferro M, Fuxe K, Agnati LF
Journal
Mol Ther Nucleic Acids
Abstract
Development of biofluid-based molecular diagnostic tests for cancer is an important step towards tum (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Characterization: Particle analysis
EM
EM-type
cryo EM
Image type
Close-up
|
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1 - 3 of 3 |
EV-TRACK ID | EV130139 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cerebrospinal fluid | Serum | Blood plasma |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C | (d)(U)C Filtration | |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 29 | 0 | 0 |