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You searched for: EV130134 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130134 | 1/1 | Gram-negative bacteria | Indoor dust |
(d)(U)C Filtration UF |
Kim YS | 2013 | 29% | |
Study summaryFull title
All authors
Kim YS, Choi EJ, Lee WH, Choi SJ, Roh TY, Park J, Jee YK, Zhu Z, Koh YY, Gho YS, Kim YK
Journal
Clin Exp Allergy
Abstract
BACKGROUND: Many bacterial components in indoor dust can evoke inflammatory pulmonary diseases. Bact (show more...)
EV-METRIC
29% (70th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Indoor dust
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
139.8 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Gram-negative bacteria
Sample Type
Indoor dust
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
45Ti
Pelleting: adjusted k-factor
139.8
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV130134 |
---|---|
species | Gram-negative bacteria |
sample type | Indoor dust |
condition | NAY |
separation protocol | (d)(U)C Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 29 |