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You searched for: EV130112 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130112 | 1/4 | Mus musculus | NAY | (d)(U)C | Romancino DP | 2013 | 22% | |
Study summaryFull title
All authors
Romancino DP, Paterniti G, Campos Y, De Luca A, Di Felice V, d'Azzo A, Bongiovanni A
Journal
FEBS Lett
Abstract
Several cell types secrete small membranous vesicles that contain cell-specific collections of prote (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Beta-enolase/ Alix/ CD63/ HSP70
non-EV: Elongin C/ Bax/ MyHc/ Myogenin/ MyoD/ PARP/ Ozz/ Bcl2 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63/ HSP70/ Beta-enolase
Detected contaminants
"Elongin C/ MyHc/ Myogenin/ MyoD/ Bcl2/ Bax/ PARP/ Ozz"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-enolase
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
Alix
Image type
Close-up
|
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EV130112 | 2/4 | Mus musculus | NAY | (d)(U)C | Romancino DP | 2013 | 22% | |
Study summaryFull title
All authors
Romancino DP, Paterniti G, Campos Y, De Luca A, Di Felice V, d'Azzo A, Bongiovanni A
Journal
FEBS Lett
Abstract
Several cell types secrete small membranous vesicles that contain cell-specific collections of prote (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Beta-enolase/ Alix/ CD63/ HSP70
non-EV: Elongin C/ Bax/ MyHc/ Myogenin/ MyoD/ PARP/ Ozz/ Bcl2 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63/ HSP70/ Beta-enolase
Detected contaminants
"Elongin C/ MyHc/ Myogenin/ MyoD/ Bcl2/ Bax/ PARP/ Ozz"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-enolase
Characterization: Particle analysis
None
|
||||||||
EV130112 | 3/4 | Mus musculus | NAY | (d)(U)C | Romancino DP | 2013 | 22% | |
Study summaryFull title
All authors
Romancino DP, Paterniti G, Campos Y, De Luca A, Di Felice V, d'Azzo A, Bongiovanni A
Journal
FEBS Lett
Abstract
Several cell types secrete small membranous vesicles that contain cell-specific collections of prote (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Beta-enolase/ Alix/ CD63/ HSP70
non-EV: Elongin C/ Bax/ MyHc/ Myogenin/ MyoD/ PARP/ Ozz/ Bcl2 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63/ HSP70/ Beta-enolase
Detected contaminants
"Elongin C/ MyHc/ Myogenin/ MyoD/ Bcl2/ Bax/ PARP/ Ozz"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-enolase
Characterization: Particle analysis
None
|
||||||||
EV130112 | 4/4 | Mus musculus | NAY | (d)(U)C | Romancino DP | 2013 | 22% | |
Study summaryFull title
All authors
Romancino DP, Paterniti G, Campos Y, De Luca A, Di Felice V, d'Azzo A, Bongiovanni A
Journal
FEBS Lett
Abstract
Several cell types secrete small membranous vesicles that contain cell-specific collections of prote (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Beta-enolase/ Alix/ CD63/ HSP70
non-EV: Elongin C/ Bax/ MyHc/ Myogenin/ MyoD/ PARP/ Ozz/ Bcl2 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63/ HSP70/ Beta-enolase
Detected contaminants
"Elongin C/ MyHc/ Myogenin/ MyoD/ Bcl2/ Bax/ PARP/ Ozz"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-enolase
Characterization: Particle analysis
None
|
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1 - 4 of 4 |
EV-TRACK ID | EV130112 | |||
---|---|---|---|---|
species | Mus musculus | |||
sample type | Cell culture | |||
cell type | NAY | |||
condition | NAY | |||
separation protocol | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C |
Exp. nr. | 1 | 2 | 3 | 4 |
EV-METRIC % | 22 | 22 | 22 | 22 |