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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV130106	1/1	Rattus norvegicus/rattus	NAY	(d)(U)C DC	Mu W	2013	22%
Study summary Full title Host matrix modulation by tumor exosomes promotes motility and invasiveness All authors Mu W, Rana S, Zöller M Journal Neoplasia Abstract Exosomes are important intercellular communicators, where tumor exosomes (TEX) severely influence he (show more...)Exosomes are important intercellular communicators, where tumor exosomes (TEX) severely influence hematopoiesis and premetastatic organ cells. With the extracellular matrix (ECM) being an essential constituent of non-transformed tissues and tumors, we asked whether exosomes from a metastatic rat tumor also affect the organization of the ECM and whether this has consequences on host and tumor cell motility. TEX bind to individual components of the ECM, the preferential partner depending on the exosomes' adhesion molecule profile such that high CD44 expression is accompanied by hyaluronic acid binding and high ?6?4 expression by laminin (LN) 332 binding, which findings were confirmed by antibody blocking. TEX can bind to the tumor matrix already during exosome delivery but also come in contact with distinct organ matrices. Being rich in proteases, TEX modulate the ECM as demonstrated for degradation of collagens, LNs, and fibronectin. Matrix degradation by TEX has severe consequences on tumor and host cell adhesion, motility, and invasiveness. By ECM degradation, TEX also promote host cell proliferation and apoptosis resistance. Taken together, the host tissue ECM modulation by TEX is an important factor in the cross talk between a tumor and the host including premetastatic niche preparation and the recruitment of hematopoietic cells. Reorganization of the ECM by exosomes likely also contributes to organogenesis, physiological and pathologic angiogenesis, wound healing, and clotting after vessel disruption. (hide) EV-METRIC 22% (59th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin NAY Focus vesicles exosomes Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (d)(U)C DC Protein markers EV: MMP14/ MMP13/ ADAM10/ uPAR/ ADAM17/ ADAMTS1/ SDF1/ CD49b/ ADAMTS8/ bFGF/ TF/ CD29/ CD44/ CD11/ HGF/ CD49c/ CD104/ TGF-alpha/ CD13/ MMP9/ MMP2/ MMP3 non-EV: Proteomics no Show all info Study aim Function Sample Species Rattus norvegicus/rattus Sample Type Cell culture supernatant EV-harvesting Medium serum free Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Below or equal to 800 g Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed Yes Pelleting: time(min) 90 Wash: volume per pellet (ml) 10 Characterization: Protein analysis Western Blot Antibody details provided? No Detected EV-associated proteins CD11/ CD29/ CD49b/ CD49c/ CD104/ uPAR/ MMP2/ MMP3/ MMP9/ MMP13/ MMP14/ ADAM10/ ADAM17/ ADAMTS1/ ADAMTS8/ CD13/ CD49c/ CD44/ bFGF/ HGF/ SDF1/ TF/ TGF-alpha ELISA Antibody details provided? No Detected EV-associated proteins CD11/ CD29/ CD49b/ CD49c/ CD104/ uPAR/ MMP2/ MMP3/ MMP9/ MMP13/ MMP14/ ADAM10/ ADAM17/ ADAMTS1/ ADAMTS8/ CD13/ CD49c/ CD44/ bFGF/ HGF/ SDF1/ TF/ TGF-alpha

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EV-TRACK ID	EV130106
species	Rattus norvegicus/rattus
sample type	Cell culture
cell type	NAY
condition	NAY
separation protocol	(d)(U)C DC
Exp. nr.	1
EV-METRIC %	22