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You searched for: EV130022 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130022 | 1/3 | Homo sapiens Mus musculus |
Cell culture supernatant | DG (d)(U)C |
Zhu H | 2013 | 78% | |
Study summaryFull title
All authors
Zhu H, Guariglia S, Yu RY, Li W, Brancho D, Peinado H, Lyden D, Salzer J, Bennett C, Chow CW
Journal
Mol Biol Cell
Abstract
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small inte (show more...)
EV-METRIC
78% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: CD63/ Hsc70/ SIMPLE/ Flotilin1/ Alix/ HSP70
non-EV: Tubulin Proteomics
no
EV density (g/ml)
1.130
TEM measurements
40-100
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens / Mus musculus
Sample Type
Cell culture supernatant
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ Flotilin1/ HSP70/ Hsc70/ SIMPLE
Detected contaminants
Tubulin
ELISA
Detected EV-associated proteins
Hsc70/ SIMPLE
Characterization: Particle analysis
NTA
EM
EM-type
immune EM/ scanning EM
Proteïns
SIMPLE
Image type
Close-up, Wide-field
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EV130022 | 2/3 | Homo sapiens | CMT1C patient B cells | DG (d)(U)C |
Zhu H | 2013 | 56% | |
Study summaryFull title
All authors
Zhu H, Guariglia S, Yu RY, Li W, Brancho D, Peinado H, Lyden D, Salzer J, Bennett C, Chow CW
Journal
Mol Biol Cell
Abstract
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small inte (show more...)
EV-METRIC
56% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
CMT1C patient B cells
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG + (d)(U)C
Protein markers
EV: SIMPLE/ Alix/ Clathrin HC/ CD63
non-EV: Tubulin Proteomics
no
TEM measurements
>100
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
CMT1C patient B cells
Separation Method
Differential ultracentrifugation
centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Obtain an EV pellet :
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
1
Density medium
Sucrose
Lowest density fraction
0.25
Highest density fraction
2.5
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ "SIMPLE/ Clathrin HC"
Detected contaminants
Tubulin
ELISA
Detected EV-associated proteins
"SIMPLE/ Clathrin HC"
Characterization: Particle analysis
NTA
EM
EM-type
scanning EM
Image type
Wide-field
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EV130022 | 3/3 | Mus musculus | Blood plasma | Microfluidics | Zhu H | 2013 | 0% | |
Study summaryFull title
All authors
Zhu H, Guariglia S, Yu RY, Li W, Brancho D, Peinado H, Lyden D, Salzer J, Bennett C, Chow CW
Journal
Mol Biol Cell
Abstract
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small inte (show more...)
EV-METRIC
0% (median: 25% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DNF
Focus vesicles
exosomes
Separation protocol
Separation protocol
Microfluidics
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
NTA
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