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You searched for: EV120084 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120084 | 1/2 | Aggregatibacter actinomycetemcomitans | Bacteria |
(d)(U)C DG Filtration |
Rompikuntal PK | 2012 | 33% | |
Study summaryFull title
All authors
Rompikuntal PK, Thay B, Khan MK, Alanko J, Penttinen AM, Asikainen S, Wai SN, Oscarsson J
Journal
Infect Immun
Abstract
Aggregatibacter actinomycetemcomitans is implicated in aggressive forms of periodontitis. Similarly (show more...)
EV-METRIC
33% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
184.6 (pelleting) / 184.6 (washing)
Protein markers
EV: LtxA/ CdtA/ OmpA/ CdtB
non-EV: DNaK Proteomics
no
Show all info
Study aim
Function
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 50,000 g and 100,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
184.6
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
184.6
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
45
Orientation
Bottom-up
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Detected contaminants
DNaK
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Characterization: Particle analysis
EM
EM-type
atomic force EM
Image type
Wide-field
|
||||||||
EV120084 | 2/2 | Aggregatibacter actinomycetemcomitans | Bacteria |
(d)(U)C DG Filtration |
Rompikuntal PK | 2012 | 33% | |
Study summaryFull title
All authors
Rompikuntal PK, Thay B, Khan MK, Alanko J, Penttinen AM, Asikainen S, Wai SN, Oscarsson J
Journal
Infect Immun
Abstract
Aggregatibacter actinomycetemcomitans is implicated in aggressive forms of periodontitis. Similarly (show more...)
EV-METRIC
33% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
184.6 (pelleting) / 184.6 (washing)
Protein markers
EV: LtxA/ CdtA/ OmpA/ CdtB
non-EV: DNaK Proteomics
no
Show all info
Study aim
Function
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 50,000 g and 100,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
184.6
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
184.6
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
45
Orientation
Bottom-up
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Detected contaminants
DNaK
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Characterization: Particle analysis
EM
EM-type
atomic force EM
Image type
Wide-field
|
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1 - 2 of 2 |
EV-TRACK ID | EV120084 | |
---|---|---|
species | Aggregatibacter actinomycetemcomitans | |
sample type | Bacteria | |
condition | NAY | |
separation protocol | (d)(U)C DG Filtration | (d)(U)C DG Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 33 | 33 |