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You searched for: EV120050 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120050 | 2/2 | Mus musculus | Semen |
(d)(U)C DG |
Nolte-'t Hoen EN | 2012 | 44% | |
Study summaryFull title
All authors
Nolte-'t Hoen EN, van der Vlist EJ, Aalberts M, Mertens HC, Bosch BJ, Bartelink W, Mastrobattista E, van Gaal EV, Stoorvogel W, Arkesteijn GJ, Wauben MH
Journal
Nanomedicine
Abstract
Nanosized cell-derived membrane vesicles are increasingly recognized as therapeutic vehicles and hig (show more...)
EV-METRIC
44% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Adj. k-factor
253.9 (pelleting)
Protein markers
EV: CD9
non-EV: Proteomics
no
EV density (g/ml)
1.1-1.18
Show all info
Study aim
Technical
Sample
Species
Mus musculus
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
253.9
Density gradient
Lowest density fraction
0.4
Highest density fraction
2
Orientation
Bottom-up
Rotor type
SW60
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
|
||||||||
EV120050 | 1/2 | Mus musculus | NAY |
(d)(U)C DG |
Nolte-'t Hoen EN | 2012 | 43% | |
Study summaryFull title
All authors
Nolte-'t Hoen EN, van der Vlist EJ, Aalberts M, Mertens HC, Bosch BJ, Bartelink W, Mastrobattista E, van Gaal EV, Stoorvogel W, Arkesteijn GJ, Wauben MH
Journal
Nanomedicine
Abstract
Nanosized cell-derived membrane vesicles are increasingly recognized as therapeutic vehicles and hig (show more...)
EV-METRIC
43% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Adj. k-factor
276.6 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.09-1.17
Show all info
Study aim
Technical
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW40
Pelleting: adjusted k-factor
276.6
Density gradient
Lowest density fraction
0.4
Highest density fraction
2
Orientation
Bottom-up
Rotor type
SW60
Speed (g)
100000
Characterization: Particle analysis
NTA
|
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1 - 2 of 2 |
EV-TRACK ID | EV120050 | |
---|---|---|
species | Mus musculus | |
sample type | Semen | Cell culture |
cell type | NA | NAY |
medium | EV Depleted | |
condition | NAY | NAY |
separation protocol | (d)(U)C DG | (d)(U)C DG |
Exp. nr. | 2 | 1 |
EV-METRIC % | 44 | 43 |