Search > Results
You searched for: EV120035 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120035 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Lv LH | 2012 | 33% | |
Study summaryFull title
All authors
Lv LH, Wan YL, Lin Y, Zhang W, Yang M, Li GL, Lin HM, Shang CZ, Chen YJ, Min J
Journal
J Biol Chem
Abstract
Failure of immune surveillance related to inadequate host antitumor immune responses has been sugges (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
232.5 (pelleting) / 232.5 (washing)
Protein markers
EV: CD63/ HSP90/ GAPDH/ HSP70/ AChE/ HSP60
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
232.5
Wash: Rotor Type
SW41
Wash: adjusted k-factor
232.5
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP90/ HSP70/ HSP60/ GAPDH/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HSP60/ GAPDH/ AChE
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV120035 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C Filtration |
Exp. nr. | 1 |
EV-METRIC % | 33 |