Search > Results
You searched for: EV120003 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120003 | 1/2 | Trypanosoma cruzi | Protozoa |
(d)(U)C DG Filtration |
Bayer-Santos E | 2012 | 78% | |
Study summaryFull title
All authors
Bayer-Santos E, Aguilar-Bonavides C, Rodrigues SP, Cordero EM, Marques AF, Varela-Ramirez A, Choi H, Yoshida N, da Silveira JF, Almeida IC
Journal
J Proteome Res
Abstract
Microorganisms use specialized systems to export virulence factors into host cells. Secretion of eff (show more...)
EV-METRIC
78% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Protozoa
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
240.8 (pelleting)
Protein markers
EV: FCaBP/ GP82/ GP35/50
non-EV: Proteomics
yes
EV density (g/ml)
1.08-1.14
Show all info
Study aim
Omics
Sample
Species
Trypanosoma cruzi
Sample Type
Protozoa
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TH641
Pelleting: adjusted k-factor
240.8
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
200000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
GP82/ GP35/50/ FCaBP
ELISA
Antibody details provided?
No
Detected EV-associated proteins
GP82/ GP35/50/ FCaBP
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV120003 | 2/2 | Trypanosoma cruzi | Protozoa |
(d)(U)C DG Filtration |
Bayer-Santos E | 2012 | 78% | |
Study summaryFull title
All authors
Bayer-Santos E, Aguilar-Bonavides C, Rodrigues SP, Cordero EM, Marques AF, Varela-Ramirez A, Choi H, Yoshida N, da Silveira JF, Almeida IC
Journal
J Proteome Res
Abstract
Microorganisms use specialized systems to export virulence factors into host cells. Secretion of eff (show more...)
EV-METRIC
78% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Protozoa
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
240.8 (pelleting)
Protein markers
EV: FCaBP/ GP82/ GP35/50
non-EV: Proteomics
yes
EV density (g/ml)
1.14-1.2
Show all info
Study aim
Omics
Sample
Species
Trypanosoma cruzi
Sample Type
Protozoa
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
960
Pelleting: rotor type
TH641
Pelleting: adjusted k-factor
240.8
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
200000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
GP82/ GP35/50/ FCaBP
ELISA
Antibody details provided?
No
Detected EV-associated proteins
GP82/ GP35/50/ FCaBP
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
1 - 2 of 2 |
EV-TRACK ID | EV120003 | |
---|---|---|
species | Trypanosoma cruzi | |
sample type | Protozoa | |
condition | NAY | |
separation protocol | (d)(U)C DG Filtration | (d)(U)C DG Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 78 | 78 |