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You searched for: EV110081 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110081 | 3/3 | Homo sapiens | NAY |
(d)(U)C DG |
Clayton A | 2011 | 33% | |
Study summaryFull title
All authors
Clayton A, Al-Taei S, Webber J, Mason MD, Tabi Z
Journal
J Immunol
Abstract
Extracellular adenosine is elevated in cancer tissue, and it negatively regulates local immune respo (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: TSG101/ CD39/ GAPDH/ CD73
non-EV: Proteomics
no
EV density (g/ml)
1.1-1.2
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.2
Highest density fraction
2.5
Orientation
Top-down
Speed (g)
150000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ CD39/ GAPDH/ CD73
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD39/ GAPDH/ CD73
|
||||||||
EV110081 | 1/3 | Homo sapiens | NAY |
(d)(U)C DC IAF |
Clayton A | 2011 | 0% | |
Study summaryFull title
All authors
Clayton A, Al-Taei S, Webber J, Mason MD, Tabi Z
Journal
J Immunol
Abstract
Extracellular adenosine is elevated in cancer tissue, and it negatively regulates local immune respo (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC IAF Protein markers
EV: TSG101/ CD39
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Immunoaffinity capture
Selected surface protein(s)
CD9, CD39, CD73
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ CD39
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD39
|
||||||||
EV110081 | 2/3 | Homo sapiens | Pleural fluid |
(d)(U)C DC |
Clayton A | 2011 | 0% | |
Study summaryFull title
All authors
Clayton A, Al-Taei S, Webber J, Mason MD, Tabi Z
Journal
J Immunol
Abstract
Extracellular adenosine is elevated in cancer tissue, and it negatively regulates local immune respo (show more...)
EV-METRIC
0% (median: 25% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Pleural fluid
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Pleural fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
|
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1 - 3 of 3 |
EV-TRACK ID | EV110081 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | Cell culture | Pleural fluid |
cell type | NAY | NAY | NA |
medium | EV Depleted | EV Depleted | |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C DG | (d)(U)C DC IAF | (d)(U)C DC |
Exp. nr. | 3 | 1 | 2 |
EV-METRIC % | 33 | 0 | 0 |