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You searched for: EV110018 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110018 | 1/3 | Homo sapiens | Saliva |
(d)(U)C DG |
Keller S | 2011 | 44% | |
Study summaryFull title
All authors
Keller S, Ridinger J, Rupp AK, Janssen JW, Altevogt P
Journal
J Transl Med
Abstract
BACKGROUND: Exosomes are small membrane vesicles with a size of 40-100 nm that are released by diffe (show more...)
EV-METRIC
44% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Annexin1/ ADAM10/ CD9/ CD24
non-EV: Proteomics
no
EV density (g/ml)
1.08-1.14
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD24/ Annexin1/ ADAM10
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24/ Annexin1/ ADAM10
|
||||||||
EV110018 | 2/3 | Homo sapiens | Amniotic fluid |
(d)(U)C DG |
Keller S | 2011 | 44% | |
Study summaryFull title
All authors
Keller S, Ridinger J, Rupp AK, Janssen JW, Altevogt P
Journal
J Transl Med
Abstract
BACKGROUND: Exosomes are small membrane vesicles with a size of 40-100 nm that are released by diffe (show more...)
EV-METRIC
44% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Amniotic fluid
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Annexin1/ ADAM10/ CD9/ CD24
non-EV: Proteomics
no
EV density (g/ml)
1.08-1.14
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Amniotic fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD24/ Annexin1/ ADAM10
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24/ Annexin1/ ADAM10
|
||||||||
EV110018 | 3/3 | Homo sapiens | Urine |
(d)(U)C DG |
Keller S | 2011 | 44% | |
Study summaryFull title
All authors
Keller S, Ridinger J, Rupp AK, Janssen JW, Altevogt P
Journal
J Transl Med
Abstract
BACKGROUND: Exosomes are small membrane vesicles with a size of 40-100 nm that are released by diffe (show more...)
EV-METRIC
44% (80th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: ADAM10/ HSP70/ CD9/ CD24
non-EV: Proteomics
no
EV density (g/ml)
1.08-1.14
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ HSP70/ CD24/ ADAM10
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24/ ADAM10
|
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1 - 3 of 3 |
EV-TRACK ID | EV110018 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Saliva | Amniotic fluid | Urine |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C DG | (d)(U)C DG | (d)(U)C DG |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 44 | 44 | 44 |