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You searched for: EV100064 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV100064 | 1/3 | Homo sapiens | NAY |
(d)(U)C SEC UF |
Szajnik M | 2010 | 22% | |
Study summaryFull title
All authors
Szajnik M, Czystowska M, Szczepanski MJ, Mandapathil M, Whiteside TL
Journal
PLoS One
Abstract
BACKGROUND: Tumor-derived microvesicles (TMV) or exosomes are present in body fluids of patients wit (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
SEC UF Protein markers
EV: MHC2/ LAMP1/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
LAMP1/ MHC1/ MHC2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP1/ MHC1/ MHC2
|
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EV100064 | 2/3 | Homo sapiens | Ascites |
(d)(U)C SEC |
Szajnik M | 2010 | 0% | |
Study summaryFull title
All authors
Szajnik M, Czystowska M, Szczepanski MJ, Mandapathil M, Whiteside TL
Journal
PLoS One
Abstract
BACKGROUND: Tumor-derived microvesicles (TMV) or exosomes are present in body fluids of patients wit (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Ascites
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
SEC Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
|
||||||||
EV100064 | 3/3 | Homo sapiens | Serum |
(d)(U)C SEC |
Szajnik M | 2010 | 0% | |
Study summaryFull title
All authors
Szajnik M, Czystowska M, Szczepanski MJ, Mandapathil M, Whiteside TL
Journal
PLoS One
Abstract
BACKGROUND: Tumor-derived microvesicles (TMV) or exosomes are present in body fluids of patients wit (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
SEC Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
|
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1 - 3 of 3 |
EV-TRACK ID | EV100064 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | Ascites | Serum |
cell type | NAY | NA | NA |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C SEC UF | (d)(U)C SEC | (d)(U)C SEC |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 22 | 0 | 0 |