EV-TRACK

Search > Results

You searched for: EV100063 (EV-TRACK ID)

Showing 1 - 1 of 1

Results list Study Tree

Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV100063	1/1	Mus musculus	BALF	(d)(U)C DC	Shin TS	2010	13%
Study summary Full title Extracellular vesicles are key intercellular mediators in the development of immune dysfunction to allergens in the airways All authors Shin TS, Kim JH, Kim YS, Jeon SG, Zhu Z, Gho YS, Kim YK Journal Allergy Abstract BACKGROUND: Previous evidence indicates that inhalation of lipopolysaccharide (LPS)-containing with (show more...)BACKGROUND: Previous evidence indicates that inhalation of lipopolysaccharide (LPS)-containing with allergens induced mixed Th1 and Th17 cell responses in the airways. Extracellular vesicles (EVs) are nanometer-sized spherical, lipid-bilayered structures and are recently in the public eye as an intercellular communicator in immune responses. OBJECTIVE: To evaluate the role of EVs secreted by LPS inhalation in the development of airway immune dysfunction in response to allergens. METHODS: Extracellular vesicles in bronchoalveolar lavage fluids of BALB/c mice were isolated and characterized 24 h after applications to the airway of 10 ?g of LPS for 3 days. To evaluate the role of LPS-induced EVs on the development of airway immune dysfunction, in vivo and in vitro experiments were performed using the isolated LPS-induced EVs. RESULTS: The inhalation of LPS enhanced EVs release into the BAL fluid, when compared to the application of PBS. Airway sensitization with allergens and LPS-induced EVs resulted in a mixed Th1 and Th17 cell responses, although that with allergens and PBS-induced EVs induced immune tolerance. In addition, LPS-induced EVs enhanced the production of Th1- and Th17-polarizing cytokines (IL-12p70 and IL-6, respectively) by lung dendritic cells. Moreover, the immune responses induced by the LPS-induced EVs were blocked by denaturation of the EV-bearing proteins. CONCLUSION: These data suggest that EVs (especially, the protein components) secreted by LPS inhalation are a key intercellular communicator in the development of airway immune dysfunction to inhaled LPS-containing allergens. (hide) EV-METRIC 13% (46th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type BALF Sample origin NAY Focus vesicles extracellular vesicles Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (d)(U)C DC Protein markers EV: CD81/ MHC2/ ICAM1 non-EV: Proteomics no Show all info Study aim Function Sample Species Mus musculus Sample Type BALF Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Below or equal to 800 g Between 800 g and 10,000 g Pelleting performed No Characterization: Protein analysis Western Blot Antibody details provided? No Detected EV-associated proteins CD81/ ICAM1/ MHC2 ELISA Antibody details provided? No Detected EV-associated proteins ICAM1/ MHC2 Characterization: Particle analysis EM EM-type transmission EM Image type Wide-field

				1 - 1 of 1

EV-TRACK ID	EV100063
species	Mus musculus
sample type	BALF
condition	NAY
separation protocol	(d)(U)C DC
Exp. nr.	1
EV-METRIC %	13