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You searched for: EV100046 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV100046 | 2/3 | Homo sapiens | Urine | (d)(U)C | Merchant ML | 2010 | 33% | |
Study summaryFull title
All authors
Merchant ML, Powell DW, Wilkey DW, Cummins TD, Deegens JK, Rood IM, McAfee KJ, Fleischer C, Klein E, Klein JB
Journal
Proteomics Clin Appl
Abstract
PURPOSE: The purpose of this study was to address the hypothesis that small vesicular urinary partic (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
104.8 (pelleting)
Protein markers
EV: AQP2/ NHE3/ CD10
non-EV: Albumin Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Equal to or above 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
110
Pelleting: rotor type
45Ti
Pelleting: adjusted k-factor
104.8
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD10/ NHE3/ AQP2
Detected contaminants
Albumin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD10/ NHE3/ AQP2
|
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EV100046 | 1/3 | Homo sapiens | Urine |
(d)(U)C UF |
Merchant ML | 2010 | 25% | |
Study summaryFull title
All authors
Merchant ML, Powell DW, Wilkey DW, Cummins TD, Deegens JK, Rood IM, McAfee KJ, Fleischer C, Klein E, Klein JB
Journal
Proteomics Clin Appl
Abstract
PURPOSE: The purpose of this study was to address the hypothesis that small vesicular urinary partic (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: AQP2/ NHE3/ CD10
non-EV: Albumin Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD10/ NHE3/ AQP2
Detected contaminants
Albumin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD10/ NHE3/ AQP2
|
||||||||
EV100046 | 3/3 | Homo sapiens | Urine |
(d)(U)C Filtration UF |
Merchant ML | 2010 | 25% | |
Study summaryFull title
All authors
Merchant ML, Powell DW, Wilkey DW, Cummins TD, Deegens JK, Rood IM, McAfee KJ, Fleischer C, Klein E, Klein JB
Journal
Proteomics Clin Appl
Abstract
PURPOSE: The purpose of this study was to address the hypothesis that small vesicular urinary partic (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: Ezrin/ AQP2/ Neprilysin/ LAMP2/ CD10/ NHE3
non-EV: Albumin Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD10/ NHE3/ AQP2/ LAMP2/ Neprilysin/ Ezrin
Detected contaminants
Albumin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD10/ NHE3/ AQP2/ LAMP2/ Neprilysin/ Ezrin
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
Neprilysin;M6P receptor;LAMP2; Tf-receptor
Image type
Wide-field
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1 - 3 of 3 |
EV-TRACK ID | EV100046 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Urine | ||
condition | NAY | ||
separation protocol | (d)(U)C | (d)(U)C UF | (d)(U)C Filtration UF |
Exp. nr. | 2 | 1 | 3 |
EV-METRIC % | 33 | 25 | 25 |